reflectance infrared atr ir spectroscopy  (JASCO Inc)

Journal: NPJ biofilms and microbiomes

Article Title: Nutrient availability influences E. coli biofilm properties and the structure of purified curli amyloid fibers.

doi: 10.1038/s41522-024-00619-0

Figure Lengend Snippet: Fig. 3 | Characterization of the structure of pur- ified curli fibers. a ThioT fluorescence emission spectra of the fibers at equivalent mass concentra- tions. b Values describing the increase in intensity of ThioT when bound to the purified fibers. Quantifi- cation of the increase was estimated by division of the area under the curve of each spectra of the probe with each fiber by the area under the curve of the emission spectra of the probe alone. The statistical analysis was done with One-way ANOVA (p < 0.001, *** | p < 0.01, ** | p < 0.05, * | ns = non- significant), where the 1.5% w/v nutrient con- centration condition was used as reference for the post-test multicomparisons. c Representative Amide I’ region of an ATR-FTIR spectrum for curli fibers. Each peak assigned to the different secondary component is highlighted in their respective color. d Distribution of the three types of secondary structure in the curli fibers obtained in the different growth conditions. The data was obtained from the Amide I’ region of each spectra. The statistical analysis was done with One-way ANOVA (p < 0.0001, **** | p < 0.001, *** | p < 0.01, ** | p < 0.05, * | ns = non-significant), where the 1.5% w/ v nutrient concentration condition was used as reference for the post-test multicomparisons.

Article Snippet: Attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR) IR spectra were acquired with a spectrophotometer (Vertex 70 v, Bruker Optik GmbH, Germany) equipped with a single reflection diamond reflectance accessory continuously purged with dry air to reduce water vapor distortions in the spectra.

Techniques: Concentration Assay